FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386275
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA116123.0061484693547342No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT112732.918387159407158No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC91522.3692964856643584No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG91052.3571289884149893No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA88962.303022458093327No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT85622.216555562746748No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA80782.0912562293702672No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC79822.0664034690311306No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC75971.9667335447543848No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA74071.9175457899165103No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT73491.9025305805449484No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT73241.896058507539965No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC69281.7935408711410263No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC68821.7816322568118568No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC66531.7223480680862082No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC66241.714840463400427No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA62831.6265613876124523No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA61171.583586822859362No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC60261.560028477121222No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA55371.4334347291437448No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA51791.340754643712381No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT48681.2602420555303864No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA45991.1906025499967638No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC42251.0937803378422108No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG41281.068668694582875No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC41231.0673742799818782No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA38160.9878972234806809No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA37770.9778007895929065No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA35070.9079024011390848No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA34130.8835674066403469No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA33210.8597501779820077No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29560.7652579121092485No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29260.7574914245032683No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA27580.7139990939097793No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT25420.6580803831467219No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT24780.6415118762539642No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT23440.6068215649472526No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA20550.5320044010096434No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA20290.5252734450844606No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT19270.49886738722412793No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC18750.48540547537376216No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT16290.42172027700472464No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT14690.38029900977283027No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC14410.3730502880072487No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14250.3689081612840593No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCGCCAAACCCCCACAAAAC14200.36761374668306257No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT13720.3551873665134943No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT13190.34146657174292927No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA13190.34146657174292927No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA9300.24076111578538606No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA9160.23713675490259528No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC9010.23325351109960518No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC8650.22393372597242894No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8620.22315707721183095No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC8580.22212154553103358No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA8150.21098957996246195No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCACTCTAACCACCCTCG7530.19493883891010289No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCACAA7430.1923500097081095No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCGCCCCCTAA7160.18536017086272732No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA7030.18199469290013592No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA7020.18173580997993657No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATGCC6720.17396932237395638No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT6520.16879166396996959No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA6470.1674972493689729No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACGT6150.159212995922594No Hit
TCCAACCAATAACACTATCTCTAACCTCGGTTTCCTCATCTGTCCTATAA5660.14652773283282636No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA5540.14342113779043428No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5530.14316225487023493No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT5310.13746683062584947No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5310.13746683062584947No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5190.1343602355834574No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCCTCTAACACC5180.13410135266325804No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA5150.13332470390266002No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA4920.1273703967380752No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4910.1271115138178759No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC4910.1271115138178759No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC4820.1247815675360818No Hit
AAAAAAATCCTTCCAACCAATAAAAACGACAACTAACCAAATAAATAACT4760.12322827001488576No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC4530.11727396285030095No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4440.1149440165685069No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA4110.10640088020192867No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCACACTCCCTTCTAACACC3880.10044657303734386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)