FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367637
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA94472.569654305741805No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG94182.56176608992022No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT93812.5517018145616466No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG93022.530213226633881No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT91732.4951242665999342No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT82292.23834924123524No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC78482.1347144057861422No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT76002.0672565601394854No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA75602.056376262454541No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA71021.9317968539619244No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG70881.927988749772194No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT70271.9113962958026531No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT68131.853186703188199No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA65671.7862728724257895No Hit
GTTGGTTTTTTTGGGGTGAAGCGGTTTTTATTTTCGTTGATGAACGGTTT62361.6962384090828724No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG59411.6159962136564054No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG55671.5142654303021732No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT55071.497944983774756No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATCGTTTTATTAGGATAGTT52751.4348392572020772No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG50941.3856059101777025No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA50471.3728215603978924No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT47761.2991075435823922No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT45781.2452500700419165No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT44851.21995337792442No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT42891.166639919268191No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG36520.9933711786354474No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG36150.9833069032768738No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT36030.9800428139713901No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT35390.9626343376754788No Hit
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGTGGGGTAATAA35260.9590982409278718No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT34430.9365216232316117No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT33560.912856975766857No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT33020.8981685738921817No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGATTTTTTGT32920.8954484994709456No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGCGGGGGGTAT31130.8467591673308181No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG28160.765972957020104No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG24260.6598900545918936No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT22560.6136487894308787No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT22310.6068486033777883No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA20910.5687675614804821No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG20100.5467349586684692No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT18740.5097419465396573No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT18520.5037577828129377No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT16680.4537084134621923No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT15620.424875624597089No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTACGATAAGGTATGTTA15420.41943547575461665No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT15260.4150833566806388No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGCGGGGGGTAT15110.41100324504878455No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT15100.4107312376066609No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT14870.4044750664378177No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT14790.4022990069008288No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA14640.3982188952689746No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT14180.3857065529312882No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTCGTGGTGGGGGGTAT13740.3737382254778491No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT13350.363129935235028No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT13230.3598658459295446No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC12900.35088960033946526No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGCGGGGGGTAT12660.3443614217284985No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT12390.3370172207911608No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTTGGGGCGTAGGCGGCGTTGAT12000.3264089305483398No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG11340.3084564393681811No Hit
TTTAAGGTTTGTGTTGATGTTAATGTTATAGGTTATGATAAGGTATGTTA11340.3084564393681811No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT11270.3065523872733158No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGTGTGAAGGTT10570.28751186632466263No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTTGGGGGTTTA9710.2641192263020316No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTCGGGGCGTAGGCGGCGTTGAT9520.25895108490168295No Hit
GGGGATATTTAGGTTTGTTTTTTTTTGGGTTTGGTGTTTTTGGAGTTTTG9280.2524229062907161No Hit
GGGTTTAGGTTGTTGAGTTTTGGTGGGAAGGGTATTTTTTTTCGTTTATC9050.24616673512187293No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGATTTTTTGT8870.24127060116364785No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT8470.23039030347870318No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT8170.22223008021499466No Hit
GATTGTTTAGGGAGGGGGTTAATAGTGTGTTAGTATTGAGGTTAGGTTTG7880.2143418643934098No Hit
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTTGGAAGTATGGTATTT7880.2143418643934098No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTCCGTTGGGGTTT7810.2124378122985445No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG7790.21189379741429726No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG6850.1863250978546773No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG6700.18224498622282306No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGTGTCGGGTA5920.16102840573718097No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTCGTGGCGGGGGGTAT5910.16075639829505736No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT5680.1545002271262142No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT5540.15069212293648354No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT5040.13709175083030273No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC4760.12947554245084147No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTTGGAGGAGAGG4740.1289315275665942No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT4730.1286595201244706No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTCGT4550.1237633861662455No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTTGTATTGTGTTATGGATGTTTT4430.12049929686076212No Hit
AGGTATTAGGGAGTTGTATGGGGTACATGGTTCTACAAGGTATTAGGGAG4410.11995528197651488No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT4390.11941126709226764No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT4330.11777922243952595No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA4320.11750721499740233No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTGTT4290.1166911926710315No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAGGATATTTAGTATTTTAGTGGTA4290.1166911926710315No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGTGGCGTTGAT4160.11315509592342446No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATCG4120.11206706615492999No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA4080.11097903638643554No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT3990.10853096940732299No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT3990.10853096940732299No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGGTTTTATTGGTTGGAAGGATTT3920.10662691731245766No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTGTAGAGGGTTCGTTGGGGTTTT3830.10417885033334512No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCAGGTA3720.10118676846998532No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)