FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300870
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG236717.867517532489115No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG160675.340180144248347No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA134954.485325888257387No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG127524.238375378070263No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT120404.001728321201848No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT108713.613188420247947No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT107603.576295409977731No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT107373.568650912354173No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG102843.4180875461162628No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG95133.161830690996111No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT72522.4103433376541363No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT49841.6565293980789046No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT47261.5707780769102935No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT46091.531890849868714No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG43451.44414531192874No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT40991.362382424302855No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT36521.2138132748363082No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG35381.1759231561804102No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA35111.1669491807092764No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGCGTTGAT30541.015056336623791No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT27390.9103599561272311No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT25500.847542127829295No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG25470.8465450194436135No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGTGATGGGTTAG22680.7538139395752318No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC22550.7494931365706119No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTGTTTTGTGATGGGTTAG20780.690663741815402No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG19920.6620799680925317No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTTTGTATGGTATAGTTTT19640.652773623159504No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGCAGGCGGCGTTGAT18570.6172100907368631No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT18570.6172100907368631No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGTGGTGTTGAT16700.5550570013627149No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG16470.5474125037391564No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT16440.5464153953534748No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG16030.5327882474158274No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT14090.4683085718084222No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGCGGCGTTGAT13390.44504270947585334No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGACATTTAGTATTTTAGTGGTA12480.4147970884435138No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG11790.3918635955728388No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT11720.3895370093395819No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGTGTTATTTTGTGATGGGTTAG11460.380895403330342No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT11330.3765746003257221No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT10010.33270183135573506No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT9760.324392594808389No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA9660.3210689001894506No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGTGTAGGCGGCGTTGAT8610.2861701066905973No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT8540.28384352045734035No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTAGAGGTTTTTTG8030.2668926779007545No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC7990.26556320005317907No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTCGT7960.26456609166749756No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTCGTT7570.25160368265363775No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT6740.224017017316449No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTTGTTGGGGTTT6490.21570778076910294No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAATATTGAGGTTAGGTTTG6460.2147106723834214No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT5860.19476850466979095No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT4970.1651876225612391No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT4220.14025991291920098No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT4180.1389304350716256No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT3810.1266327649815535No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTTAATTAATTGAGAAGTT3790.12596802605776583No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG3260.10835244457739224No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTACGTTGGAGT3250.10802007511549838No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTTGAGTTTGGTTTTTTTTTATT3240.10768770565360455No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT3170.10536111942034765No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT3140.10436401103466612No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGAGTGTAGGTGGTGTTGAT3120.10369927211087845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)