FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300870
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA227147.549439957456709No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT220117.315784225745338No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC167835.578156678964337No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT144544.804068202213581No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC139514.636886362880978No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT124194.127696347259613No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA117413.9023498520955893No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA104163.4619603150862504No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA83292.768305248113803No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA71892.389404061554824No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA65492.176687605942766No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC65472.176022867018978No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT60192.0005317911390303No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC44591.482035430584638No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT35921.1938711071226775No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC34621.1506630770764783No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG30321.0077442084621266No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC28280.9399408382357829No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC26300.8741316847808024No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC21420.7119353873766079No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC20340.676039485492073No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC19270.640475953069432No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC19250.6398112141456443No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA18380.6108950709608801No Hit
CAACTACCAAACCCTAAAAAACCACAAACACTCAACACCAACCCATAAAA17120.5690165187622561No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC15740.523149533020906No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC14100.4686409412703161No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT13870.46099644364675774No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC12200.40549074351048625No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC11830.3931930734204141No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA10740.35696480207398545No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9920.32971050619869047No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC9580.3184099444942999No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC9190.30544753548044007No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA9080.3017914713996078No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT9010.2994648851663509No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7520.24994183534416858No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA7350.2442915544919733No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6800.22601123408781204No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA6320.21005749991690764No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCCAAAAATTTCTAAAAT5950.1977598298268355No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC5760.19144481005085254No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA5470.18180609565593114No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAAACCTACCGCCAAAAAAAC5450.18114135673214343No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA5060.16817894771828365No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA4240.14092465184298866No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA4040.13427726260511183No Hit
ACTCCCACACACACTAACACAATAAACAACGCTACGAACATCAATACACT3880.1289593512148104No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA3540.11765878951041978No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA3420.11367035596769369No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA3230.1073553361917107No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCA3130.1040316415727723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)