FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325126
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA233727.188597651372083No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC172385.30194447691049No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT158364.870727041208639No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT155124.771073368478683No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA131514.0448933644187175No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA127663.9264777347858986No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT126063.8772660445488825No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC114423.5192509980745927No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC101943.135399814225869No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA92302.838899380547849No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA87822.701106647884205No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA76552.3544718047772246No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC68682.1124118034239032No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT65021.9998400620067296No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA35771.1001888498612844No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC35231.0835799044062917No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG35051.0780435892546274No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC32861.010685088242712No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC30530.9390205643350578No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29020.892577031673874No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC28020.8618197252757392No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT26450.8135307542306675No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT22900.7043423165172886No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA20410.6277566235859329No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA13420.41276305186297No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA12610.3878496336804808No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA11520.35432416970651376No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11480.3530938774505884No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA10660.3278728862041178No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA10260.31556996364486384No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC9570.29434742223015076No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA9220.2835823649908036No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT9160.28173692660691546No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8520.2620522505121091No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7910.2432902936092469No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7500.2306797979860116No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7480.2300646518580489No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC7410.22791164041017944No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA7260.2232980444504592No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA6720.2066890989954664No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC6410.19715433401204457No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5860.18023781549307039No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA5640.1734712080854807No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT5390.165781881485947No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAGA4880.1500956552228982No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT4570.1405608902394764No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC4500.13840787879160693No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA4370.13440942895984942No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAGCCTTATT4330.133179136703924No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4300.13225641751197997No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC4180.1285655407442038No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA3780.11626261818494983No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3680.11318688754513634No Hit
CCTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC3670.112879314481155No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG3650.1122641683531923No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAAGC3460.10642028013754669No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA3390.10426726868967723No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT3370.10365212256171454No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)