FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360169
Sequences flagged as poor quality0
Sequence length301
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT118063.27790564984771No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA115053.1943337710907933No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT101582.820342672467647No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG84442.344454964197363No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC82682.2955890151567737No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA80812.2436689443011475No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC78932.1914712260077907No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT78742.1861959246909093No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA78072.167593546362957No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC76202.1156734755073314No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT74832.0776357765382363No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC70031.9443650064275384No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA68661.906327307458443No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC63781.7708353578459002No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA61991.7211364664921192No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA61961.720303524178927No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA59061.639785767237047No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA58091.6128539657771768No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC49201.3660253936346547No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA48251.3396488870502459No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA44751.2424722838445286No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG43971.22081578370154No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC42461.1788910206042165No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA41171.1430745011369663No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA39961.109479161171561No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC39411.094208552096377No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC36961.0261849298523749No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA32470.901521230311326No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAGGCGACCATCAAACA32380.8990224033717504No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT32320.8973565187453668No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT31300.8690364800968434No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC29800.8273893644372502No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29530.8198928836185235No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA27530.7643633960723993No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT26970.7488151395594846No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT26040.7229939278505368No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC24730.6866221135078254No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA24640.6841232865682498No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24230.6727397416212945No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC23500.6524714786669592No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCACCAAACCCCCACAAAAC23040.6396996965313506No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC21870.6072149463168679No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC21530.5977749334340268No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA21470.596109048807643No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA20820.5780619653551528No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA19830.5505748690198212No Hit
AATACCTTAACTCCCTAAAACCCAAACATAAATCAAAAATATCCCAAAAC19720.5475207472047845No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17830.4950453814736971No Hit
CATAATCACCCAATCTAAAACCAAAAACCTACTGTAGAACCATGTCGTCA16050.4456241375576465No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA15900.44145942599168725No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC15180.42146881047508256No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT14840.4120287975922414No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCA14070.3906499448869836No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA11840.32873456627305514No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTGAATACACGACC11510.3195722008279447No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC10680.29652746349630316No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC10480.29097451474169067No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9740.27042860434962473No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT9040.2509932837084813No Hit
AAACTACAAAATCAAAACCAACCCCACGTACCCCATACAACTCCCTAATA8950.2484944567689057No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA8010.22239559762222733No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC7420.2060143987961207No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC7130.1979626231019327No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6920.19213202690958966No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC6420.17824965502305862No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC6190.17186376395525435No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC5400.14992961637453528No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC4640.1288284111070081No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA4340.12049898797508948No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4140.11494603922047705No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3980.11050368021678712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)