FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316172
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA230827.30045671343446No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC179025.662107966549853No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC174015.503649912073175No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT165775.243032273572612No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT135134.273939501284111No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT134554.255595055855673No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA123883.9181205166807938No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA110863.5063193451665553No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA106863.3798059284187087No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT86212.7266804144579533No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA73542.325949166909151No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC72292.286413724175449No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA68952.180775021190997No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA46761.478941841782321No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA43211.3666611844186076No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC36221.1455789886517465No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG34061.0772617436079097No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC29340.9279759118454513No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC28340.8963475576584896No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT25480.8058904646837797No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT24620.7786900800829928No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC21160.669255974596106No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC20750.6562883493794517No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC19860.628139114153056No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA18860.5965107599660944No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC18720.5920827903799197No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA15820.5003605632377314No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC14230.4500714800804625No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC13630.43109446756828557No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12330.3899776071252356No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC11740.37131687815492836No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA11350.35898182002201334No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA10480.3314651518793568No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7870.2489151474513872No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC7790.2463848791164303No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA7440.23531495515099377No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC6850.21665422618068644No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT6810.21538909201320802No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC6390.20210518325468416No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA5540.17522108219576685No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACA5240.1657325759396784No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACACCCTAA4870.15403008489050266No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA4850.15339751780676342No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4700.14865326467871917No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3650.11544349278240958No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACGTCAATACAC3510.11101552319623495No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAAACCTACCACCAAAAAAAC3500.11069923965436534No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA3420.10816897131940842No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCA3210.1015270169401465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)