FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316699
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA203846.436395441728582No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC158385.000963059561287No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT154114.866134720981121No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC153874.858556547384109No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT152124.803299031572566No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT110763.4973271150208873No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA109343.452489587905235No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA105183.321134578890366No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC75312.377967723295621No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA74652.3571277459038393No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA67322.1256776939617743No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA58671.8525476872361453No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA57421.813078033085043No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT52941.6716187926074915No Hit
CCCAAAAATTAATAAATAAACCCCACTCACCTACTTAATCTCCCTGTAGA44251.3972257569490272No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG41471.3094452461169754No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT35231.1124127325946718No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC34751.0972563854006487No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT33981.0729430784435694No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC33941.0716800495107341No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC32361.0217904066637407No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29070.917906276938039No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC22680.7161374049176031No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA22510.7107695319530533No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC21410.676036236300083No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC18650.5888872399344488No Hit
AAAAAAAATCCCAAAACAAACATACGAAACCAAAAAAATACCTAAAATAA17620.5563642449139403No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC16020.5058430876005292No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA15520.49005522594008827No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA13940.4401655830930947No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA13850.43732376799421535No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC13140.41490500443638917No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC12620.3984856283095305No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA12520.3953280559774423No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC11930.37669837921812194No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAACTTCTAAAAT11840.37385656411924256No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC10780.3403862973991077No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTCATT9960.31449420427598446No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC8890.2807081803226408No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC8300.2620785035633204No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA7820.246922156369297No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA7710.243448826804No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA6640.2096628028506563No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6490.20492644435252402No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT6310.19924281415476525No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA6100.19261191225738003No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA5940.1875597965260389No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCCATCCTATAA5940.1875597965260389No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5580.1761925361305214No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC5440.17177193486559791No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC5380.16987739146634503No Hit
CATAATCACCCACAACAAATTTCCCACCGCCAAAATAAACCACCCCCTAA5070.1600889172368716No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA4820.1521949864066511No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA4750.14998468577418939No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA4540.14335378387680414No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA4360.1376701536790454No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA4200.13261803794770427No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT4090.12914470838240727No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3960.12503986435069261No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTCCTAAAAT3870.12219804925181323No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC3600.1136726039551751No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCGAACA3330.10514715865853697No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCCAACCGAAACCCAAAAAA3200.10104231462682231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)