FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250242
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA165156.599611575994438No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA148665.940649451331112No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT134405.370801064569497No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC127495.094668361026526No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC123484.934423478073225No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA122874.91004707443195No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT114794.5871596294786645No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT76033.038258965321569No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC74802.9891065448645713No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA64362.571910390741762No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA62782.5087715091791147No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT59682.384891425100503No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA33181.3259165128155945No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT32961.3171250229777576No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAACTTCTAAAAT31891.2743664133119141No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC31281.249990009670639No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC26451.0569768464126725No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC21060.8415853453856666No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA20750.8291973369778055No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC15300.6114081569041168No Hit
AAAACAAAAAAAACTCCTAATCCCTACAACCTCAAAACGACCATCAAACA15270.6102093173807754No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC14250.5694487735871676No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGGAACCCAAAAAA13180.5266901639213242No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG12720.508307957896756No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC12680.5067095051989674No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC12420.4963195626633419No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA11860.47394122489430235No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA11310.45196250029970986No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC9480.3788332893758841No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC9150.3656460546191287No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCGCCCCCTAA9020.3604510833513159No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC8360.334076613837805No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA8330.3328777743144636No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCGCACCAACAT8010.32009015273215524No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC7820.3124975024176597No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT7600.3037060125798227No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6800.2717369586240519No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT6340.2533547525994837No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC6130.24496287593609384No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC6110.24416364958719958No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAAACCTACCGCCAAAAAAAC5880.2349725465749155No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA5550.22178531181816No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA4990.19940697404912047No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACGT4740.18941664468794206No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAACAA4590.18342244707123503No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC4180.1670383069189025No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA3480.13906538470760305No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA3220.12867544217197752No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3190.1274766026486361No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC3130.1250789236019533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)