FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262888
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG209847.982106448373452No Hit
GATAGGTTTGGAGATAGTTTTGTTTAAAGATATTTAGTATTTTAGTGGTA157695.9983719302516665No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG136045.174827302881836No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG133955.095325766105718No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG118824.519795502267125No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT118594.511046529320471No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT101483.8601990201150302No Hit
TGGGATATTTTTGATTTATATTTGGGTTTTAGGGAGTTAAGGTATTGTGG77312.9407960804601196No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT65932.5079121146648No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGTGTTGAT51701.9666169623565928No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG50561.9232524877514379No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT48011.826253005082012No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTCGCGATGGGTTAG45241.7208849395940478No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT37651.4321688323544628No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT37371.4215179087672316No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT36901.4036395727458082No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT34851.325659596482152No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT30521.160950671008186No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT27211.0350415386019902No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGCGATGGGTTAG24110.9171205988862178No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGCGTAGGCGGTGTTGAT20420.7767566416116369No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTTGTTATTTTACGATGAGTTAG20100.7645841575119442No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGCAGGTGGTGTTGAT18890.7185569520099814No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGTGGCGTTGAT17680.6725297465080186No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA17050.6485651684367487No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAC15710.5975928912692858No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT14840.5644989501232464No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG14200.5401539819238611No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGTGTAGGCGGTGTTGAT12880.48994248501262894No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT12690.48271507257843643No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGCGCGTTATTTTGCGATGGGTTAG12460.4739660996317824No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTTGGGGTGTAGGTGGTGTTGAT12270.4667386871975899No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT11550.43935059797328135No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG11290.4294604546422811No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG11130.4233742125924348No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGTGTTTATTTTTTTT11000.4184291409269346No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT10860.41310367913331913No Hit
GGTTGTAGGAGGTTTTTAGTAAGGGAGGGGTTGTAGGAGTGTTTTTGGGG10820.4115821186208576No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT10770.40968016798028056No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTGCGTTATTTTGTGATGGGTTAG9950.37848817747481817No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT8420.3202884878731627No Hit
GATAGGTTTGGAGATAGTTTTGTTTTTGTGGTTGTGTAGGATATAGTTTC7050.2681750403213536No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT6800.2586652871184687No Hit
GAATTTTGGGTTTGATTTTTGTTTCGGTTTGGGGCGTAGGCGGCGTTGAT6530.24839475365935304No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTTGGGGCGTAGGCGGCGTTGAT6320.24040656096892973No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT6110.23241836827850643No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAATATTGAGGTTAGGTTTG5780.21986549405069838No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT5530.21035574084781353No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTTGT4180.15900307355223517No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGGGGTTAGGTTTG3870.1472109795806579No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTCGTGTAGAGGGTTCGTTGGGGTTT3850.14645019932442713No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATACGTTGTTGGTTGTTTTGA3770.14340707829950397No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGAATGAAGGCGTGAAGGTT3530.13427771522473447No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGACGTTGAGCGTGAAGAAGTGG3160.12020328048446487No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTTGGGGTAAGCGTTTATTTTTTTT3100.1179209397157725No Hit
TAGGTTTTATTGTGAGGATGATTTGGGTGATATGTTGTTGGTTGTTTTGA3020.11487781869084933No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTCGAATTTTTTTTTTTGTT2950.11221508779404156No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTAATTAATTGAGAAGTTGTTTTT2930.11145430753781078No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTTAATTAATTGAGAAGTT2780.10574845561607985No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT2670.10156416420681051No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)