FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262888
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA199167.575849791546211No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT196957.491783573232707No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA147605.614558290983233No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC130424.961048050880983No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC123674.704284714403092No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT106284.042786281610419No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT101603.864763701652415No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA70012.6631112869358815No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA62732.3861872736678738No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT61732.3481482608563344No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC56782.1598551474392136No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA51801.970420863637747No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT44351.6870302181917776No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC43381.650132375764584No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA36171.3758710933933842No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAACTTCTAAA35571.3530476857064604No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA32001.2172484099692646No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC29441.1198685371717234No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC20240.7699096193055598No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT19630.7467058214905207No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC17670.6721493563799033No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC16420.6246005903654788No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC16060.6109065457533246No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC12390.4713033687349746No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC11600.4412525486138584No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC11240.4275585040017041No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT11210.426417333617358No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG11170.4248957731048964No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC8310.31610419646389337No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA8030.3054532728766623No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC7990.30393171236420075No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC7130.27121816134627674No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7050.2681750403213536No Hit
CAACTACCAAACCCTAAAAAACCACACACTCAATACCAACCATAAAAACA6720.25562216609354554No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC6140.23355953866285262No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC5720.21758315328200603No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT5580.2122576914883905No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAAACCTACCGCCAAAAAAAC5200.19780286662000549No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA4950.1882931134171206No Hit
ACTCCCACACACACTAACACAATAAACAACGCTACGAACATCAATACACT4800.18258726149538967No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA4570.1738382885487356No Hit
GCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT4460.16965399713946624No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA3960.15063449073369647No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC3650.13884239676211924No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAAATTAACTATATC3170.12058367061258027No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC2980.11335625817838776No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2970.11297586805027236No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAACTTCTAAAAT2900.1103131371534646No Hit
GCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC2770.10536806548796446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)