FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354296
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA221886.262560119222345No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA202545.71668887032312No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT186545.265089078059025No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC164614.646115112787048No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT155904.400275475873281No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT148334.186612324158331No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA118463.3435319619752977No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC112403.1724885406552716No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA104382.9461241447828934No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC102502.893061169191862No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA94642.6712127712421254No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC90822.5633933208390722No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA77362.183484995596902No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT66031.8636958926998892No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT52451.4804005690157382No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA40771.1507327206629485No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG37091.0468647684422066No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC32830.9266263237518911No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC32100.9060220832298417No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC31710.8950143382934045No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC28820.8134441258157021No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA24590.6940524307358819No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC23290.6573599476144241No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT22310.6296994603382482No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC20170.5692979881229254No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC19900.5616772416284689No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC17790.5021225190236412No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC16460.46458328629168827No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA14350.4050285636868607No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAGGCGACCATCAAACA12820.36184433355160656No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC12560.354505836927315No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA11810.3333370966649355No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11090.3130151060130512No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT10910.30793460835008013No Hit
CAACTACCAAACCCTAAAAAACCACTCTACACTAACCGAAACCCAAAAAA10070.28422561925621515No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC9560.2698308758777971No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA9040.255153882629214No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC8710.24583963691376703No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7790.21987264885858152No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA7440.2099939034028044No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA7270.20519565560999845No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA6930.1955991600243864No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAAACCTACCGCCAAAAAAAC6910.19503466028405628No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5770.16285817508523948No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5640.15918892677309368No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA5380.15185043014880215No Hit
CCTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC5020.14168943482285998No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA4970.1402781854720347No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC4760.13435093819856844No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA4610.13011719014609252No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA4450.12560119222345156No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)