FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344328
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA213616.203677888524895No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT206946.009967240538091No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC163164.738505146255895No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT150864.381287609488627No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT145414.223008294416951No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA122553.559106433400711No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA106523.0935619525568647No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA95712.7796171092679076No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA95052.7604493390023466No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC90522.628888733997816No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA83412.422399572500639No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC80072.3253990381264376No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT69272.01174461559908No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC64831.882797797448944No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACAAACATCAATACAC47391.3763039892195814No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA38821.127413396528891No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT37601.0919820636137636No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG34100.9903347970539718No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC33870.9836551195371854No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC32530.9447387374828652No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC31210.9064031969517437No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC25940.7533514555888571No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC25470.7397016797936852No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT23600.6853929973745964No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC22020.6395065170418903No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAGCGACCATCAAACA21250.6171441183987361No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC19110.5549940754164634No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA17240.5006853929973746No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC15730.45683185799586445No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC14560.422852628888734No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA12490.3627355312376571No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCCAAAAT12440.3612834274296601No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC12410.36041216514486185No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC11530.3348551381241142No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10950.3180107339513487No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT10550.3063939034873725No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA10370.3011663297785832No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA9610.2790943518970284No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC7960.23117492623312658No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA7750.22507609023953903No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7700.22362398643154202No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA7590.2204293580539486No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC7410.2152017843451593No Hit
AAAACAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACAA7320.21258799749076462No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC7100.2061987407355777No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA6660.19342022722520388No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC6010.17454287772124252No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAAACCTACCGCCAAAAAAAC5280.15334216212448595No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA4660.13533607490532285No Hit
CAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA4620.1341743918589252No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA4340.12604261053414187No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA4110.11936293301735555No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3880.11268325550056922No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC3630.1054227364605841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)