FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000888714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84318680
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2825800.33513332988609407No Hit
GGCAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGC1887970.22390886574600077No Hit
CAGAGACAGAGGCGGCGGCCCGGGGGATCCGGTACCCCCAAGGCACGCCT1446430.1715432452215808No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1313590.1557887291404467TruSeq Adapter, Index 19 (97% over 40bp)
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG872460.10347173366565986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)