FastQCFastQC Report
Fri 15 Sep 2023
EGAF00000901352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57207766
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCCGTGAGAATCTCGTAT3703750.6474208414291164Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGAAGTAATCTCGTAT2801540.4897132322908746TruSeq Adapter, Index 7 (97% over 37bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCGAAGTAATCTCGTAT1754760.306734578658429Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1383060.24176088260464498Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAACTCCAGTCACCCGTGAGAATCTCGTATGCCGTCTTCTGCTT1340850.23438251373074068RNA PCR Primer, Index 19 (96% over 30bp)
CAAGCAGAACTCCAGTCACCCGAAGTAATCTCGTATGCCGTCTTCTGCTT1086290.18988505861249677Illumina PCR Primer Index 5 (96% over 27bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACCCGTGAGAATCTCGTAT1012190.17693227174786025Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)