FastQCFastQC Report
Fri 24 Mar 2023
EGAF00000901358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71956594
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCACTTCGAATCTCGTAT2819020.39176673648560967Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGCGTTAATCTCGTAT2526830.3511603120069858Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGTTAATCTCGTAT2507540.3484795292006178TruSeq Adapter, Index 10 (97% over 37bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT819460.11388254424604922Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACCAGCGTTAATCTCGTAT815910.11338919126716865Illumina Single End PCR Primer 1 (95% over 22bp)
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG721110.10021458214100572No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)