FastQCFastQC Report
Thu 9 Feb 2023
EGAF00000901360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133832338
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCACTTCGAATCTCGTAT5059550.378051379480496Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAGCGTTAATCTCGTAT4573300.3417186061563088Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTTCGAATCTCGTAT3560810.26606499245346815TruSeq Adapter, Index 7 (97% over 35bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACCAGCGTTAATCTCGTAT1484280.11090593067274966Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)