FastQCFastQC Report
Fri 17 Mar 2023
EGAF00000901362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85241114
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCAATGGAAATCTCGTAT4640860.5444391540917685Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGGAAATCTCGTAT2955650.3467399546186128TruSeq Adapter, Index 2 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAAGACTAATCTCGTAT1437410.1686287206429517Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACCAATGGAAATCTCGTAT1246710.14625688725748Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)