FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000901364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118627675
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACCAATGGAAATCTCGTAT6588390.5553838933452923Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTAT2937620.24763361500594194TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTAATCTCGTA2229490.18794012442712038TruSeq Adapter, Index 2 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAAGACTAATCTCGTAT2028780.1710208010061733Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACCAATGGAAATCTCGTAT1768590.1490874705249007Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)