FastQCFastQC Report
Sat 13 May 2023
EGAF00000901366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34995060
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT16652014.758388755441483Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT3487860.9966721017194998Illumina Single End PCR Primer 1 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT2065600.5902547388117065TruSeq Adapter, Index 23 (97% over 37bp)
AATGATACGGCCACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT1035580.29592176724371955Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACAACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT982880.28086249887841314Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAACCACAATCTCGTAT921500.2633228804294092Illumina Single End PCR Primer 1 (96% over 25bp)
AATCATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT842280.24068539959640017Illumina Single End PCR Primer 1 (95% over 22bp)
ATTGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT824860.23570755415192887Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCGCGAACTCCAGTCACATTGAGGAATCTCGTAT762260.21781931506904118Illumina Single End PCR Primer 1 (95% over 22bp)
AATAATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT749720.21423595216010488Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCAACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT636860.18198568597967826Illumina Single End PCR Primer 1 (95% over 22bp)
AACGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT557820.15939964097789802Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGCCCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT551890.15770511609352864Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCCACCACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT499230.14265727791293972No Hit
GATGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT452750.12937540327120456Illumina Single End PCR Primer 1 (95% over 22bp)
GCTTGGAACTCCAGTCACCAACCACAATCTCGTATGCCGTCTTCTGCTTG414610.11847672214306819Illumina PCR Primer Index 1 (96% over 28bp)
AGTGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT403110.11519054403678691Illumina Single End PCR Primer 1 (95% over 22bp)
AAGGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT402440.11499908844276878Illumina Single End PCR Primer 1 (95% over 22bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACCACAATCTCGTAT376570.10760661647672556TruSeq Adapter, Index 7 (97% over 35bp)
AATGATACGGCGACAACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT370720.10593495196179119No Hit
AATCATACGGCGACCACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT359810.10281736908009302No Hit
AATGATACGGCGACCACCAAGAACTCCAGTCACATTGAGGAATCTCGTAT353030.10087995277047676Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)