FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000901368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38691148
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT18381554.75084120016289Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT6099961.5765776709442687Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT5843751.5103583899862574TruSeq Adapter, Index 25 (97% over 38bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT3997031.0330605853307842Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCCAGATCGGAAGAGCACACGTCTGAACTCCAGT1446610.3738865541027627Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCCACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT1128080.2915602297455739Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACAACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT1103010.28508071148470443Illumina Single End PCR Primer 1 (95% over 22bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1099950.2842898329095844No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT1097690.2837057199750186TruSeq Adapter, Index 27 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCAACCACAATCTCGTAT937350.24226471646692935Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGCGAACTCCAGTCACATTGAGGAATCTCGTAT880170.22748614230831302Illumina Single End PCR Primer 1 (95% over 22bp)
AATCATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT878520.22705968817466984Illumina Single End PCR Primer 1 (95% over 22bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT820710.21211828607411698TruSeq Adapter, Index 27 (97% over 36bp)
AATAATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT816970.2111516567045258Illumina Single End PCR Primer 1 (95% over 22bp)
ATTGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT743210.19208786464542227Illumina Single End PCR Primer 1 (95% over 22bp)
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG720320.18617178275506324No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG713740.1844711353615044No Hit
AATGATACGGCAACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT693670.1792839023540992Illumina Single End PCR Primer 1 (95% over 22bp)
GATGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT635260.1641874260231307Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGCCCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT621160.16054318160836167Illumina Single End PCR Primer 1 (95% over 22bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC598430.15466845284611352No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT577360.1492227627880155No Hit
AATGATACGGCCACCACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT566560.14643142664053288No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTAT564190.14581888343039084TruSeq Adapter, Index 27 (97% over 36bp)
GTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTG507840.13125482862384957No Hit
AGTGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT491730.127091085537188Illumina Single End PCR Primer 1 (95% over 22bp)
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT479950.12404646148002638No Hit
CCGGAGTAGTAAGTTACAATATGGGAGATTATTCCGAAGCCTGGTAGGAT472570.12213904844591326No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGAGGAATCTCGTA465200.12023421998230706TruSeq Adapter, Index 25 (97% over 38bp)
AAGGATACGGCGACCACCGAGAACTCCAGTCACATTGAGGAATCTCGTAT453710.11726454846984638Illumina Single End PCR Primer 1 (95% over 22bp)
CTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCTATG439340.11355052065139035No Hit
AATGATACGGCGACAACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT431190.11144409568824373No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC414600.10715629321724959No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG412720.10667039396194705No Hit
GTGAGTGTTAGGAAAAGGGCATACAGGACTAGGAAGCAGATAAGGAAAAT401430.10375241385962494No Hit
GTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCA399140.10316054721353836No Hit
AATCATACGGCGACCACCCAGAACTCCAGTCACATTGAGGAATCTCGTAT398880.10309334838035822No Hit
GCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAAT398740.10305716439326122No Hit
AATGATACGGCGACCACCAAGAACTCCAGTCACATTGAGGAATCTCGTAT397580.10275735421445753Illumina Single End PCR Primer 1 (95% over 22bp)
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT390810.10100759998126704No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)