FastQCFastQC Report
Mon 3 Apr 2023
EGAF00000901369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38691148
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT4836751.250092139938572Illumina Single End PCR Primer 1 (100% over 31bp)
GGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT1266070.3272247181706782Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC677960.17522354208771473Illumina Single End PCR Primer 1 (100% over 50bp)
CTAGGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATT448700.11596967864587528No Hit
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGG430430.11124766832971716Illumina Single End PCR Primer 1 (96% over 30bp)
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA418470.10815652200343086No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)