FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000901372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108286314
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACAGTCACTAATCTCGTAT3469640.3204135288971051Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCACTAATCTCGTAT3369410.3111575115577394TruSeq Adapter, Index 13 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT1556190.1437106816656443Illumina Single End PCR Primer 1 (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)