FastQCFastQC Report
Tue 14 Feb 2023
EGAF00000901382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120224282
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGTATCAATCTCGTAT4899800.4075549396918004TruSeq Adapter, Index 8 (97% over 37bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2590970.2155113723199445Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCAGATTTAATCATAATACATGAATTAAATTAATCAAATTAAGGAACAG2487890.2069373972223016No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACACGTATCAATCTCGTAT1932180.16071462169347786Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1254730.10436577196609916No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC1235940.10280285974176165No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)