FastQCFastQC Report
Fri 24 Mar 2023
EGAF00000901384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71231589
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT12708511.7841115407379162Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT4217420.5920715877895129Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT3323010.4665079140660473Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTCCAAATCTCGTAT1045300.14674669127485TruSeq Adapter, Index 8 (97% over 37bp)
AATGATACGGCGACCACCGCGAACTCCAGTCACACAGCAGAATCTCGTAT761840.10695254881931666Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACAACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT737980.10360291134316826Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)