FastQCFastQC Report
Wed 27 Sep 2023
EGAF00000901386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000901386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46808962
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGATACGGCGACCACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT7959271.7003731037658985Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT3102860.6628773353273675TruSeq Adapter, Index 5 (97% over 37bp)
AATGATACGGCGACCACCCAGAACTCCAGTCACACAGCAGAATCTCGTAT2655480.5673016205742824Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACACCTCCAAATCTCGTAT2094890.447540366308486Illumina Single End PCR Primer 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT744680.159089193219025Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA709140.15149663006840441No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC677050.1446411052652695No Hit
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT616870.13178459287347583TruSeq Adapter, Index 5 (97% over 37bp)
GTGGGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGT559540.11953693824699639No Hit
AATGATACGGCGACCACCGCGAACTCCAGTCACACAGCAGAATCTCGTAT481940.10295891628615905Illumina Single End PCR Primer 1 (95% over 22bp)
AATGATACGGCGACAACCGAGAACTCCAGTCACACAGCAGAATCTCGTAT469290.10025644234537823Illumina Single End PCR Primer 1 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)