FastQCFastQC Report
Mon 12 Sep 2022
EGAF00000902789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8209443
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3559864.336299064382321RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT2927603.5661371910371997RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2618073.1890957766562233RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2347272.859231740813597RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG1464801.7842867049567188RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1439391.753334544134115RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1041451.268600074329038RNA PCR Primer, Index 1 (100% over 28bp)
TGGAGTGTGACAATGGTGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1039481.2662003987359436RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC983841.198424789599002RNA PCR Primer, Index 1 (100% over 31bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA918321.118614259213445RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC859781.0473061327059583RNA PCR Primer, Index 1 (100% over 31bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA853851.0400827437379125No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC847541.0323964731833815RNA PCR Primer, Index 1 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA797390.9713082848617135No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT645680.7865089020046793RNA PCR Primer, Index 1 (100% over 30bp)
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA599810.7306342220781605RNA PCR Primer, Index 1 (100% over 37bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG571660.6963444413951105RNA PCR Primer, Index 1 (100% over 29bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT496190.6044137221002691RNA PCR Primer, Index 1 (100% over 35bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG495310.6033417857947244RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT484260.5898816765035094RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC407360.4962090607121579RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT402410.4901794189934689RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG401930.4895947264631717RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG384880.46882596054324266RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG356940.4347919828421977Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG355920.4335495112153164RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC355540.4330866296288311RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT339060.41301218608862983RNA PCR Primer, Index 1 (100% over 30bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC315150.38388718942320443RNA PCR Primer, Index 1 (100% over 36bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG304700.3711579457948609RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC302640.36864864035233574RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG300720.36630987023114725RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG299750.36512830407617175RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG288250.35112004553780324No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG285290.34751444160097095No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC283470.34529748242359437RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTCC277790.33837862081507847RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA276210.3364540079028504RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCA256260.31215272461237625RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC234570.28573193089957505No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG223070.2717236723612065RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC214760.26160118293043755No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC211560.25770323272845674No Hit
TGTAACAGCAACTCCATGTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG210530.25644858000719417RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC208470.25393927456466897RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG204210.24875012835828206No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG199420.24291538414969202RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC196930.2398822916487757RNA PCR Primer, Index 1 (100% over 27bp)
TGGNGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG187580.228492968402363RNA PCR Primer, Index 1 (100% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA181390.22095287098040634RNA PCR Primer, Index 1 (100% over 23bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT174200.2121946641203307RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAG171230.20857687908911723RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA168120.20478855873656715RNA PCR Primer, Index 1 (100% over 34bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC164560.2004520891368635RNA PCR Primer, Index 1 (100% over 27bp)
CTGNCCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT164440.20030591600428918RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC161030.19615216282030337RNA PCR Primer, Index 1 (100% over 44bp)
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC157360.19168170118240666RNA PCR Primer, Index 1 (100% over 31bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG156460.19058540268809954RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG153140.18654127935354445Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCAG151560.1846166664413164RNA PCR Primer, Index 1 (100% over 29bp)
TGGCGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG151240.18422687142111835RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC148490.1808770704662911RNA PCR Primer, Index 1 (100% over 31bp)
AAGNTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG146870.1789037331765383RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG143380.17465253123750296RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG142080.17306898896794826RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG141570.17244775315450755RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTGGAATTCTCGGGTGCCAAGGAACTCCAG140310.1709129352624776RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA138570.16879342484015053RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC137630.16764840196831868RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA136540.16632066268076898RNA PCR Primer, Index 1 (100% over 28bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA135740.16534617513027378RNA PCR Primer, Index 1 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA133840.16303176719784765No Hit
CGACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC128310.15629562200504954RNA PCR Primer, Index 1 (100% over 36bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC125260.15258038821878658RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC125070.15234894742554397RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA122040.14865807582804338No Hit
TCANTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG121650.148183013147177RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC120300.14653856540571633No Hit
TCAGTGCACTACAGAACTTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG119980.14614877038551824RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA118580.14444341717215162RNA PCR Primer, Index 1 (100% over 28bp)
ATGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT118150.14391963011376047RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCCA114040.13891320032309135No Hit
GGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTA112170.1366353356738088RNA PCR Primer, Index 1 (100% over 47bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA111900.13630644612551668No Hit
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA111820.13620899737046716RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCAG111260.1355268560851205RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA111250.13551467499073933RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCCA107880.13140964618427828RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA107300.13070314271016925No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT106710.12998445814167905RNA PCR Primer, Index 1 (100% over 30bp)
ACTGGACTTGGAGTCAGAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA104520.12731679847219843RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC103700.12631794873294083RNA PCR Primer, Index 1 (100% over 51bp)
CACTAGATTGTGAGCTCCTGGATGGAATTCTCGGGTGCCAAGGAACTCCAG99230.12087299954454889RNA PCR Primer, Index 1 (100% over 29bp)
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGGA98540.12003250403224677RNA PCR Primer, Index 1 (100% over 22bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG97720.11903365429298918RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC97490.1187534891222218No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCAA97420.11866822146155348No Hit
AGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC93080.113381626500117RNA PCR Primer, Index 1 (100% over 42bp)
TTCAAGTAATCCAGGATAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT91630.11161536781484445RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGGA90490.11022672305538878RNA PCR Primer, Index 1 (100% over 22bp)
CACTAGATTGTGAGCTCCTGGAATGGAATTCTCGGGTGCCAAGGAACTCCA89200.10865536188021527RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGT88240.107485976819621RNA PCR Primer, Index 1 (100% over 30bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC86900.10585371017254154RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA86090.10486704152766517RNA PCR Primer, Index 1 (100% over 32bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA84240.10261353906714499RNA PCR Primer, Index 1 (100% over 22bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG82930.10101781570320909RNA PCR Primer, Index 1 (100% over 29bp)
CATAAAGTAGAAAGCACTACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT82150.10006769034147628RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph