FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000902950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127646970
Sequences flagged as poor quality0
Sequence length59
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC46991283.6813470778037267Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC15626221.224174768895807Illumina Paired End PCR Primer 2 (100% over 40bp)
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC6208740.4863993246373181Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC6070560.4755741558142743Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3007610.2356193805462049No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAAGCGGTTCAGCAGGAATGCC2059330.16133011226196753Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT1813240.14205115875449295No Hit
GATCGGAAGAGCGGTTTAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1733350.13579249080491296Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCTGAGATCGGAAGAGCGGTTCAGCAGGAATGC1604470.12569589391741928Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGTCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1335780.10464643226548974Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)