FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000902952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130586627
Sequences flagged as poor quality0
Sequence length59
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT4261460.32633203704694813No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT2215280.1696406478130414No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC2079130.15921461850760568Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTG2045530.15664161384611No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATG1782850.13652623097463112No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGCGGGGGGGGT1477780.11316472704360454No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC1378070.10552918255557668Illumina Paired End PCR Primer 2 (100% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)