FastQCFastQC Report
Sat 11 Mar 2023
EGAF00000902953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116701203
Sequences flagged as poor quality0
Sequence length59
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC14865651.273821487512858Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC12779161.095032413676147Illumina Paired End PCR Primer 2 (100% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC3248620.27837073796060186Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3014170.25828097076257217No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT2081880.17839404791739807No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT2074300.1777445259069009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)