FastQCFastQC Report
Sat 11 Mar 2023
EGAF00000902956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134740933
Sequences flagged as poor quality0
Sequence length59
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT14025791.0409449962766697No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC11788770.8749212089840583Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC9506500.7055391252189118Illumina Paired End PCR Primer 2 (100% over 40bp)
ATTTTCAGGGATTTATAAGTTGATGATGTTATAATATTTTTGATTTTTTAAATAGTTTA6058470.44963841834166307No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA3623450.2689197647161906Illumina Paired End PCR Primer 2 (96% over 33bp)
TTTTTAGTTTAAAGATTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT3433280.2548060135519471No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT2153230.1598051870399324No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTTAGGTGGTTTTTTATTTAAGTATT1412160.10480556788188486No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)