FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000902958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132324299
Sequences flagged as poor quality0
Sequence length59
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT4527470.3421495548599128No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC3360510.2539601588971954Illumina Paired End PCR Primer 2 (100% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC3274410.24745341745585217Illumina Paired End PCR Primer 2 (97% over 36bp)
ATTTTCAGGGATTTATAAGTTGATGATGTTATAATATTTTTGATTTTTTAAATAGTTTA2084650.157540981947692No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT2033670.15368832598160975No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA1803200.13627126790975858Illumina Paired End PCR Primer 2 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)