FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000902960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122434769
Sequences flagged as poor quality0
Sequence length59
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC11217260.9161825592205757Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC8346550.6817140317388111Illumina Paired End PCR Primer 2 (100% over 40bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT4847780.3959479843507525No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT4181270.3415100166522142No Hit
ATTTTCAGGGATTTATAAGTTGATGATGTTATAATATTTTTGATTTTTTAAATAGTTTA1942210.15863222643888028No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGTAGGAGAATG1888090.15421191344756No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA1787520.1459977435004594Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTTAAAGTGTTG1692320.1382221744543823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)