FastQCFastQC Report
Thu 23 Feb 2023
EGAF00000902962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130283481
Sequences flagged as poor quality0
Sequence length59
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC31492362.4172181890043296Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC5796020.44487758198600785Illumina Paired End PCR Primer 2 (100% over 40bp)
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC5060560.38842683363672176Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC4306070.3305154242846796Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3317710.25465315898337104No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT2018270.15491373000695308No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAAGCGGTTCAGCAGGAATGCC1324910.10169439669792059Illumina Paired End PCR Primer 2 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)