FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000902963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127276948
Sequences flagged as poor quality0
Sequence length59
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC21229271.667958757150588Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC3715300.29190674810964196Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3479700.2733959334097169No Hit
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC3215720.2526553355129163Illumina Paired End PCR Primer 2 (100% over 40bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT2293760.18021802345543358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)