FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000902964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117020975
Sequences flagged as poor quality0
Sequence length59
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC33647922.875375119716786Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC4725450.40381222255240995Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC3289880.28113592456395103Illumina Paired End PCR Primer 2 (100% over 40bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3081420.26332202410721667No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT1857070.15869548172880973No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT1604360.13710020789008123No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAAGCGGTTCAGCAGGAATGCC1446190.12358382760013749Illumina Paired End PCR Primer 2 (97% over 36bp)
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1444120.1234069362351493Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTTAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1216970.10399588620757945Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)