FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000902965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124084132
Sequences flagged as poor quality0
Sequence length59
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC43926773.5400795647262937Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC7980600.6431604002355434Illumina Paired End PCR Primer 2 (100% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC7264890.5854809864004207Illumina Paired End PCR Primer 2 (100% over 30bp)
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC5676870.4575016892570921Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3660270.2949829233604181No Hit
GCTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC2737240.2205954908078013Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT2361770.19033618255072293No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAAGCGGTTCAGCAGGAATGCC1858890.14980884098862857Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1788940.14417153677635428Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTTAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1641170.13226268125887364Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCTGAGATCGGAAGAGCGGTTCAGCAGGAATGC1454040.1171817843719131Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGTCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1251900.10089122435091055Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)