FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000902966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120336907
Sequences flagged as poor quality0
Sequence length59
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC34252332.846369484966071Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC6715060.5580216549856978Illumina Paired End PCR Primer 2 (100% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC4702190.39075210733146065Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT3317070.27564860047466566No Hit
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC3183700.26456555011838556Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT2254650.18736147173867448No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT1338270.11121027067780627No Hit
GATCGGAAGAGCGGTTTAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1281300.10647606224414594Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)