FastQCFastQC Report
Wed 22 Feb 2023
EGAF00000902967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126858859
Sequences flagged as poor quality0
Sequence length59
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC38265263.0163648247853154Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC17811571.4040462085505594Illumina Paired End PCR Primer 2 (100% over 40bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC6246380.49238815871739794Illumina Paired End PCR Primer 2 (100% over 30bp)
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC5992010.4723367407868614Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT4029810.31766090533732455No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT2433190.1918029232786967No Hit
TATTTGTTGGAATTTTTTGGGGAATTTGGTATTTTCAGGTGGTTTTTTATTTAAGTATT1899890.14976407757222537No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAAGCGGTTCAGCAGGAATGCC1627170.12826617020101055Illumina Paired End PCR Primer 2 (97% over 36bp)
GCTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1524950.12020839632492675Illumina Paired End PCR Primer 2 (96% over 33bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1474590.11623863020871093Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTTAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC1438710.11341029009255081Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCTGAGATCGGAAGAGCGGTTCAGCAGGAATGC1309510.10322574318597647Illumina Paired End PCR Primer 2 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)