FastQCFastQC Report
Sat 18 Mar 2023
EGAF00000902968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111168294
Sequences flagged as poor quality0
Sequence length59
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC13115721.1798076167292808Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC8770510.7889398752489627Illumina Paired End PCR Primer 2 (100% over 40bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT2724550.2450833688245679No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC2168990.19510868809410714Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGT1912510.17203736165997116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)