FastQCFastQC Report
Tue 14 Feb 2023
EGAF00000902969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000902969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138371238
Sequences flagged as poor quality0
Sequence length59
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC13569310.9806452696477284Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATC4443270.32111225311144503Illumina Paired End PCR Primer 2 (100% over 40bp)
GGTCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGC2820910.2038653437501224Illumina Paired End PCR Primer 2 (96% over 33bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCAGGAATGCC2052790.14835380745816554Illumina Paired End PCR Primer 2 (100% over 30bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATAGGCGTTCGT1801380.13018456913712081No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)