FastQCFastQC Report
Mon 19 Dec 2022
EGAF00000923081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166621551
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT158177359.493210755192166TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA14212680.8529916997351681TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2273910.13647154202759762TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTAT2128080.1277193728679191TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTAT1958520.11754301818976587TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACAGTTCCGTATCTCGTAT1724720.10351121986615044TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)