FastQCFastQC Report
Tue 24 Jan 2023
EGAF00000923083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179191291
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT156009988.706337184657038TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA11870560.6624518375728428TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACAGTTCCGTATCTCGTAT2074350.11576176433708489TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2020960.11278226685693112TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACAGTTCCGTATCTCGTAT1820390.10158920055997588TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)