FastQCFastQC Report
Tue 24 Jan 2023
EGAF00000923108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179285651
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2009919211.210708658441384TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA5560010.31012018914999506TruSeq Adapter, Index 18 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT4041210.22540621502386712TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTCCGCACATCTCGTAT2939720.1639685040940616TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT2538500.1415896914137317TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGCACATCTCGTAT2410670.13445972873757756TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTCCGCACATCTCGTAT2327730.12983359164643912TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTCCGCACATCTCGTAT2197050.12254466477074621TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTCCGCACATCTCGTAT2133930.11902402607780363TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTAT2068030.11534832756917061TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTCCGCACATCTCGTAT2025930.11300011956896651TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCTGCACATCTCGTAT1978630.11036187162574433TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTCCGCACATCTCGTAT1866730.10412043515964364TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)