FastQCFastQC Report
Sat 1 Apr 2023
EGAF00000923143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12550269
Sequences flagged as poor quality0
Sequence length100
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC190290415.162256681510172TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2590172.063836241278972TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGNCTGAACTCCAGTCACTGACCAATCTCGTATGC879440.7007339842675882TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAACCTCGTATGC595980.47487428357113304TruSeq Adapter, Index 4 (98% over 50bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC363790.28986629688973203TruSeq Adapter, Index 4 (98% over 50bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC228390.18198016313435195TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGCCTGAACTCCAGTCACTGACCAATCTCGTATGC198660.15829142785704434TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGCCACTGACCAATCTCGTATGC196890.15688109952065568TruSeq Adapter, Index 4 (98% over 50bp)
GANCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC169320.1349134428911444TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCACTCACTGACCAATCTCGTATGC169090.1347301798869809TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTGACCAATCTCGTATGC168230.13404493561054348TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTGACCAATCTCGTATGC158260.12610088277789105TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC156380.12460290691777205TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGC139900.11147171427162239TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATTC134700.10732837678618683TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTGACCAATCTCGTATGC130250.1037826360534583TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACTGACCAATCTCGTATGC128870.10268305802847733TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)