FastQCFastQC Report
Mon 6 Mar 2023
EGAF00000923145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72918623
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC2633901336.12110585247886TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG18950642.598875187206977TruSeq Adapter, Index 4 (100% over 49bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC8065191.1060535248999421TruSeq Adapter, Index 4 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC4449840.6102473986652216TruSeq Adapter, Index 4 (98% over 50bp)
GAACGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC4191520.574821606271967TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTGACCAATCTCGTATGC3276580.44934748699245186TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3041700.41713623692537366TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC2507560.3438847165284512TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTGACCAATCTCGTATGC2251450.30876200171799734TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTGACCAATCTCGTATGC2212610.30343551605465724TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTGACCAATCTCGTATGC2072480.2842182030782452TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTGACCAATCTCGTATGC2036340.27926199319479744TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAACTCGTATGC2015280.2763738421116373TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACTAATCTCGTATGC1974330.27075799278327023TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTGACCAATCTCGTATGC1911250.2621072534515634TruSeq Adapter, Index 4 (98% over 50bp)
AGTTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1695520.23252221863816602TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACTGACCAATCTCGTATGC1495330.2050683266468156TruSeq Adapter, Index 4 (98% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1453220.1992933958722726TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1431930.19637370277823266TruSeq Adapter, Index 4 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCCATCTCGTATGC1395020.19131189572792673TruSeq Adapter, Index 4 (98% over 50bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG1089240.14937747795923134TruSeq Adapter, Index 4 (100% over 49bp)
AGGTCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG881260.12085527177330267TruSeq Adapter, Index 4 (97% over 49bp)
GATCGGAAGAGCACACGTCTGCACTCCAGTCACTGACCAATCTCGTATGC829040.11369386391182948TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)