FastQCFastQC Report
Fri 24 Mar 2023
EGAF00000923153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53501590
Sequences flagged as poor quality0
Sequence length100
%GC28

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1342614325.09484858300473TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG4280790.8001238841686761TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1892810.3537857473020895TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC1821710.3404964226296826TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC1322920.24726741765992377TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGC1268170.23703407693117157TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC1265020.23644530938239405TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC1164380.21763465347478458TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC1136620.21244602263222456TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGC1108620.2072125333097577TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC886820.1657558214625023TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)