FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000923155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142388618
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6758861547.46770911141226TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG11488060.8068102746808035TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTTGTAATCTCGTATGC9395820.6598715636105128TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC9158590.643210821808805TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTTGTAATCTCGTATGC6572230.4615698987962647TruSeq Adapter, Index 12 (98% over 50bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6510500.4572345803651244TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTGTAATCTCGTATGC6506870.4569796442578016TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTTGTAATCTCGTATGC6204430.43573918246752TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTTGTAATCTCGTATGC6126580.4302717510749349TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTTGTAATCTCGTATGC5497010.38605684058258083TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTTGTAATCTCGTATGC5441850.3821829354365951TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4304090.30227767222236823TruSeq Adapter, Index 12 (98% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC4186730.2940354403889221TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCCGTCACCTTGTAATCTCGTATGC3048710.21411191728822032TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATATCGTATGC2660190.18682602846809004TruSeq Adapter, Index 12 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC2147820.15084211295596675TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGC1807540.12694413538025912TruSeq Adapter, Index 12 (98% over 50bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1642640.11536315353520743TruSeq Adapter, Index 12 (98% over 50bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1484530.10425903564848141TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)