FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000923167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155664727
Sequences flagged as poor quality0
Sequence length100
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT144107709.257569314337989TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA6892310.44276633074363725TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTAT2735390.1757231745891926TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTAT2566530.16487550194977696TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2527780.16238617756995133TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGTAT2047170.13151148879090635TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTAT2013430.12934400996315626TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGTCAGAATCTCGTAT1878360.1206670281829486TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGTCAGAATCTCGTAT1832510.11772159533610976TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTAT1712970.11004227052670706TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATATGTCAGAATCTCGTAT1647220.1058184491596481TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)