FastQCFastQC Report
Mon 23 Jan 2023
EGAF00000923185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000923185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151381534
Sequences flagged as poor quality0
Sequence length100
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC2006459713.254322683769344TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG56065153.703565984474698TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3376500.22304569855924436TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGC3013980.19909825989740595TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGC2414550.15950096000480482TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGC2251410.14872421625744656TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGC2178680.14391979935941196TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGC2128010.1405726275702821TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGC2093700.13830616883562563TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGC2010020.1327784140435517TruSeq Adapter, Index 2 (98% over 50bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1532610.10124154244598947TruSeq Adapter, Index 2 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)